L型氨基酸转运载体1及其配体在奶牛乳腺中的协同表达研究

doi:10.11843/j.issn.0366-6964.2014.02.007

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (2): 212-217.doi: 10.11843/j.issn.0366-6964.2014.02.007

L型氨基酸转运载体1及其配体在奶牛乳腺中的协同表达研究

林叶，侯晓明^*

（东北农业大学乳品科学教育部重点实验室，哈尔滨 150030）

收稿日期:2013-08-20 出版日期:2014-02-23 发布日期:2014-02-23
通讯作者: 侯晓明，副教授，E-mail：xiaominghou@126.com
作者简介:林叶（1980-），女，黑龙江兰西人，博士，副教授，主要从事泌乳生物学与乳腺功能调控研究，E-mail：linlu516@163.com
基金资助:
2010年高等学校博士学科点专项科研基金联合资助课题（新教师类联合）（20102325120018）；2012年黑龙江省博士后科研启动金（LBH-Q12157）

Research on L-type Amino Acid Transporter 1 and Its Ligand Coordinated Expression in Dairy Cow Mammary Gland

LIN Ye，HOU Xiao-ming^*

(The Key Laboratory of Dairy Science of Ministry of Education，Northeast Agricultural University，Harbin 150030， China)

Received:2013-08-20 Online:2014-02-23 Published:2014-02-23

摘要/Abstract

摘要：

为探讨氨基酸转运与乳腺泌乳功能之间的关系，本研究采用激光扫描共聚焦显微技术对泌乳期和干奶期奶牛乳腺中LAT1和4F2hc的表达含量和表达方式进行研究。结果显示，LAT1和4F2hc的蛋白表达含量在泌乳期高乳蛋白率荷斯坦奶牛中显著高于泌乳期低乳蛋白率的荷斯坦奶牛和干奶期的荷斯坦奶牛。泌乳期LAT1和4F2hc协同表达于腺泡上皮细胞基底侧的细胞膜上和围绕导管的肌上皮细胞；干奶期LAT1和4F2hc协同表达于乳导管上皮细胞质膜。相关性分析显示泌乳期和干奶期LAT1和4F2hc在乳腺中的表达高度正相关，相关系数0810。结果表明，LAT1是奶牛乳腺中负责转运氨基酸的载体，LAT1/4F2hc协同表达与泌乳期乳蛋白生物合成以及干奶期乳腺组织重建紧密正相关。

Abstract:

To investigate the underlying relationship between amino acid transportation and lactation in dairy cow mammary gland，LAT1 and 4F2hc expression were detected by laser confocal microscope.The results showed that LAT1 and 4F2hc expression were higher in mammary gland with high quality milk than mammary gland with low quality milk and mammary gland in dry period.In lactation，LAT1 and 4F2hc were detected on the basolateral of acinar epithelial cells and myoepithelial cells.In dry period，LAT1 and 4F2hc were detected on the plasma membrane of ductal epithelial cells.Correlation analysis showed LAT1 expression was highly correlated with 4F2hc.All these results revealed that LAT1 was the major amino acid transporter in mammary gland of dairy cow.LAT1/4F2hc coordinated expression was closely related to milk protein synthesis and mammary gland reconstruction.

中图分类号:

S823.9+1.2

林叶，侯晓明. L型氨基酸转运载体1及其配体在奶牛乳腺中的协同表达研究[J]. 畜牧兽医学报, 2014, 45(2): 212-217.

LIN Ye，HOU Xiao-ming. Research on L-type Amino Acid Transporter 1 and Its Ligand Coordinated Expression in Dairy Cow Mammary Gland[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 212-217.

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L型氨基酸转运载体1及其配体在奶牛乳腺中的协同表达研究

Research on L-type Amino Acid Transporter 1 and Its Ligand Coordinated Expression in Dairy Cow Mammary Gland

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